Description
“Fluorescent in situ hybridization targeting ribonucleic acid molecules (RNA FISH) is a methodology for detecting and localizing particular RNA molecules in fixed cells. This detection utilizes nucleic acid probes that are complementary to target RNA sequences within the cell. These probes then hybridize to their targets via standard Watson-Crick base pairing, after which one may detect them via fluorescence microscopy, either through direct conjugation of fluorescent molecules to the probe, or through fluorescent signal amplification schemes. Recent advances in RNA FISH have increased the specificity and sensitivity of the method to enable the detection of individual RNA molecules, providing very accurate measurements of of RNA abundance and localization at the single cell or even subcellular level. While most applications thus far have been in fixed cells, advances in probe technology have lead to the ability to detect single RNA molecules in living cells.” (Introduction taken from [1])
Protocol
- Blah
Useful resources
- Stellaris® RNA FISH
- Stellaris – education materials & protocols
- “single molecule RNA FISH” by the Raj Lab
References