Max Planck Institute

Here you can find an overview of the research projects I have worked on between 2010 and 2015. For further information or if you have any questions, please do not hesitate to contact me.

 

4Pi-RESOLFT nanoscopy

4pi_resolft_isosted_squareFrom 2011 to 2015. I did my Ph.D. studies in the Department of NanoBiophotonics at the Max Planck Institute for Biophysical Chemistry of Prof Stefan Hell in Göttingen, Germany. In his department, physicists, biologists, chemists, and engineers conceive, invent and utilize optical microscopes with a resolution at the nanometer scale to advance life sciences.

The major technique used to achieve this goal is Stimulated Emission Depletion (STED) Microscopy. Besides STED in various forms, 4Pi Microscopy is used and further developed as well. While STED can overcome the diffraction barrier down to 5 nm in the lateral direction, 4Pi Microscopy can increase the resolution in the axial direction by using two opposing lenses.
Both techniques have already been combined successfully to create isotropic diffraction-unlimited point spread functions. Nevertheless, the usage of high laser intensities should be avoided when imaging living cells.

My doctoral research was about the development of a new super-resolution technique for the three-dimensional visualization of living biological structures and their dynamics at low light levels – 4Pi-RESOLFT nanoscopy. The developed microscopy approach combines two fluorescence microscopy techniques: REversible Saturable OpticaL Fluorescence Transitions (RESOLFT) Microscopy and 4Pi Microscopy.

My Ph.D. thesis project resulted in the following paper:

 

3D in situ imaging of block copolymers nanostructures with the isoSTED

This project resulted in the following paper:

 

Imaging synaptic vesicles release of neurotransmitter with the isoSTED

This project resulted in the following paper: