National Institutes of Health

Here you can find an overview of the research projects I have worked on between 2017-2018. For further information or if you have any questions, please do not hesitate to contact me.

 

Designing and building a microscope for live-cell 5-color single-molecule transcription imaging in eukaryotic cells

From 2017 to 2018, I worked at the Center for Cancer Research | National Cancer Institute at the National Institutes of Health. The primary goal of my host department is to understand gene expression in eukaryotic cells, starting from the mechanistic behavior of individual macromolecules and proceeding to their regulation in cells and tissue. Therefore, I was engaged in using, developing, and combining new technologies, including (1) super-resolution microscopy, (2) single-molecule imaging, (3) genetic engineering, (4) next generation sequencing approaches, and (5) mathematical and biophysical modeling. Specifically, I designed and built a microscope for live-cell 5-color single-molecule transcription imaging and used a modified CASFISH (CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells) approach to label specific loci within the nucleus of eukaryotic cells.